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Cell nuclei were counterstained by Hoechst dye blue. Silencing of the transforming growth factor-beta TGFbeta receptor II by Kruppel-like factor 14 underscores the importance of a negative feedback mechanism in TGFbeta signaling. Cancers of the gastrointestinal tract, such as colorectal tumors, frequently metastasize to the liver. Nat Cell Biol 5: Mice were killed at day 6 after tumor implantation, and their livers were snap frozen in optimal cutting temperature embedding compound for cryo-sectioning and IF analysis.

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Mechanisms of hepatic fibrogenesis. Human hepatic stellate cell lines, LX-1 and LX Shah, and a startup fund to N.

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Li X, Eriksson U. J Exp Med Data are representative of multiple experiments with consistent results.

Colocalization of these 2 proteins arrows was quantitated, and quantitative data are shown on the bottom. Ligand-dependent and -independent transforming growth factor-beta receptor recycling regulated by clathrin-mediated endocytosis and Rab Retroviral and lentiviral transduction of HSCs. Mice were killed at day 6 after tumor implantation, and their livers were snap frozen in optimal cutting temperature embedding compound for cryo-sectioning and IF analysis.

Selective upregulation of platelet-derived growth factor alpha receptors by transforming growth factor beta in scleroderma fibroblasts.

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Ammanamanchi S, Brattain MG. Clin Cancer Res Cytokine Growth Factor Rev Rheumatology 47Suppl 5: In response to tumor invasion of the liver, hepatic stellate cells HSCswhich are resident liver-specific pericytes, are activated into tumor-associated myofibroblasts that in turn promote tumor implantation and metastatic growth in the liver 14 Cell nuclei were counterstained by Hoechst dye.

Silencing of the transforming growth factor-beta TGFbeta receptor II by Kruppel-like factor 14 underscores the importance of a negative feedback mechanism in TGFbeta signaling. Cells were harvested for Western blot WB. Kang at the Hormel Institute.

The colorectal liver metastases were positive for carcinoembryonic antigen, a clinical diagnostic marker of colon and rectal cancers Supplemental Fig. Quiescent HSCs were negative for both proteins arrowheads. S1; supplemental material for this article is available online at the Journal of American Physiology Gastrointestinal dis Liver Physiology website.

Densitometry of WB is shown on the right. Two experiments were performed to test this hypothesis: Insight into the physiological functions of PDGF through genetic studies in mice.

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S10—S19, [ PubMed ]. Densitometric analysis is shown on the bottom. HT29 human colorectal cancer cells were implanted into the livers of SCID mice by portal vein injection.

Open in a separate window. Cancers of the gastrointestinal tract, such as colorectal tumors, frequently metastasize to the liver.

Arterioscler Thromb Vasc Biol Next, the same double IF technique was performed on liver biopsies of patients with colorectal cancer that were obtained from a Mayo Clinic tissue collection The prometastatic microenvironment of the liver. Author information Article notes Copyright and License information Disclaimer.

Further analysis and quantification were done as previously described 5. Nat Cell Biol 2: